ABI Applied Biosystems 7300 Real-Time PCR System with control computer

ABI Applied Biosystems


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The ABI 7300 Real-Time PCR system is an affordable solution for budget-conscious labs. The four color detection system gives users the flexibility to run a variety of applications including SNP genotyping, gene expression, plus/minus assays, pathogen quantitation and more. The instrument is equipped with a CCD camera for precision optics and comes with advanced multi-componenting algorithms for accurate and reproducible results.

Applied Biosystems 7300 Real-Time PCR specifications:

Thermal cycling system Peltier-based, 96-well block
Sample Ramp Rate +/-1.1°C/sec
Peak Block Ramp Rate 1.6°C/sec
Temperature Range 4°C – 100°C
Temperature Accuracy ± 0.25°C of setpoints / display temperature measured at 3 minutes after clock start
Temperature Uniformity ±0.50°C, 30 seconds after clock start
Optical system Single excitation, four emission filters, and CCD camera
Calibrated Dyes at Installation SYBR Green, FAM, JOE, NED, TAMRA, ROX
Passive Reference Dyes  ROX or any calibrated dye
Data Collection Data collected in all 4 filters for all wells regardless of plate setup. Plate setup may be altered after run completes
Quantitative PCR run time < 1 hour 50 minutes
Supported Volumes 20 – 100 μL
Supported Consumables  Standard optical 96 well plates
0.2ml tubes
Optical adhesive covers
Optical flat caps
8-strip 0.2ml tubes 


Seller refurbished. Passes all built in functional tests. Instrument is in excellent functional condition. Unit was removed in current configuration from a working environment. Instrument will require end user calibration prior to entering service.


Complete system, includes the following:
    • (1) ABI Biosystems 7300 Real-Time PCR System main unit (manufactured in 2005)
    • (1) Ultra Fast Dell Optiplex 9020 I5-4570 3.2Ghz (8GB High Speed RAM installed) computer with SDS 1.4 software
    • Large Dell Flatscreen monitor, keyboard, and mouse
    • USB and power cable
    • (1) SDS 1.4 application disk
    • (1) Windows 7 Professional DVD with license
    • (6) Various user manuals and guides in full color:
      • ABI 7300 Absolute Quantification Getting stated Guide
      • ABI 7300 Fast Real-Time PCR System Installation and Maintenance Guide
      • ABI 7300 Reagents Guide
      • ABI 7300 Allelic Discrimination Getting Started Guide
      • ABI 7300 Relative Quantification Getting Started Guide
      • ABI 7300 Absolute Quantitation Using Standard Curve Getting Started Guide


      With your purchase, we offer the option for custom, white glove, delivery of the instrument from our warehouse to your laboratory. Our courier will deliver and place the instrument at your desired location. This service is offered at no additional expense beyond the standard shipping charge for the item.

      Please note, our courier team is very busy delivering quality instruments with a smile! If you choose the white glove delivery option, we will schedule your item for delivery as soon as possible.


      30 Day warranty. We pledge to offer the best service and warranty to all of our customers!  We are extending a 30 Day warranty on this item. This is our way of saying thank you for allowing us to serve you.


      This is a genuine product from this manufacturer.