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Fluorescence Polarization (FP)

Fluorescence polarization measurements can provide information on the orientation and mobility of labelled macromolecules in the context of associations such as receptor-ligand interactions, protein-DNA interactions, and enzymatic catalysis.

• The Panvera Full Range Beacon 202 Variable Temp Fluorescence Polarization System provides a simple, rapid, and sensitive way to study equilibrium binding events and affinities for a range of labelled macromolecules. The instrument is configured with fluorescein filters and variable temperature control.

Highlights:
High Precision: Less than 2 mP standard deviation, at 1 nM fluorescein, for 0.1 ml sample.
High Sensitivity: Accurate to 10-11 M fluorescein in the polarization mode and 10-13 M fluorescein in the intensity mode, for 0.5 ml sample.
Variable Temperature Control: 6-65°C in 1°C increments, ± 1°C.
Sample Volumes and Standard Chamber: Sample volume 100 μl in disposable 6 mm x 50 mm glass test tubes.
Operating Software: Step-by-step prompting for measurement of blanks and samples.
Read Time: Static or Kinetic mode (minimum 6 seconds; number of read cycles variable, for very high precision.)
Programmable Protocols: Up to 99 user-defi ned protocols, password protected.
Output Signals and Printer: RS232 for interface with an IBM-compatible computer (not included); DB25 Parallel for interface with
external parallel, thermal printer (included).

Compatible Spreadsheet Programs
Microsoft® Excel, or Lotus 1-2-3, or into a plotting/curve-fitting program

Mechanical:
Sample Format: Single-tube
Overall Dimensions: 20.5" x 14.75" x 9.25" (52.6 cm x 37.8 cm x 23.7 cm)
Weight: 35 lb (15.9 kg)

Electro-Optical:
Excitation Illumination:
Standard Instrument: High-intensity 100 Watt quartz halogen lamp for 360-700 nm excitation.
Red-Range Instrument: Same as standard instrument plus 100 Watt high-intensity mercury lamp for excitation from 254-420 nm. Plus an enhanced photomultiplier tube that increases emission detection range to 900 nm.
Emission Detector: Photomultiplier tube (PMT) with selected high gain, low noise, low drift for 200-700 nm emission detection.
An enhanced PMT that increases the emission detection range to 900 nm is also available in the Red-Range Instrument.
Optics: Six-element quartz optical system focuses excitation energy on the sample and collects focused, emitted fluorescent energy on the PMT detector. Excitation and emission polarizers and fi lters are in collimated light paths for optimum performance.
Optical Filters & Holders: Fluorescein filters standard, in easily accessible, external filter holders. Filter holders accommodate other wavelength fi lters for use with different fluorophores.

Polarizers:
Standard Instrument: Film polarizers.
Red-Range Instrument: Glan-Taylor type of prism polarizer.

Condition

Received from a laboratory environment.  Good condition, sold with warranty.

LRGS

Includes

• Panvera Beacon 2000 Variable Temperature Fluoresence Polarization System
  
• CBM-1000 Printer
• Emission Filter 159374 C 479 0417  (some buildup around outer lens edge)
  
• Excitation Filter 159373 C 643 0417 (some buildup around outer lens edge)
  

Warranty

30 Day warranty. We pledge to offer the best service and warranty to all of our customers!  We are extending a 30 Day warranty on this item. This is our way of saying thank you for allowing us to serve you.

Certification

This is a genuine product from this manufacturer.

Disclaimer

Regardless of the origin of the equipment, documentation provided or identification appearing upon the equipment, the equipment described and offered here is in no way certified for, recommended for, or offered for any specific use. The purchaser agrees that the seller shall not be held responsible or liable for any injuries or damages, whether incidental or consequential, associated in any way with the equipment. The purchaser, by bidding on this equipment, indicates their acknowledgment of, and agreement to the terms of this disclaimer. it is the purchaser's responsibility to have the item inspected and certified by a qualified professional before use!